Leonardo is one of the titular heroes that appears in Teenage Mutant Ninja Turtles series, alongside Raphael, Michelangelo, and Donatello. In Alvin and the Chipmunks 2: Trouble in Los Angeles Chinatown, Leonardo and his brothers would later turned into horses by an enraged Brittany Miller (Christina Applegate) to take Alvin and Simon to the Chinatown, hoping to pursues the Purple Dragons in order to prevent them from escaping with a leather that briefcase contains a ransom Belgian euros. However, before leaving, Brittany told them that the will turned to normal after dawn. In Alvin and the Chipmunks 2: Rape and Revenge, he and his brothers once again turned into horses by Casey Jones (Joshua Peck) to take Alvin, Simon, and Theodore to the Los Angeles downtown, hoping to pursues Hun and the Purple Dragons in order to prevent them from escaping with a leather briefcase contains ransom Swiss francs. Before leaving, however, Casey Jones told the three chipmunks that the four of them will returned to their normal selves after daybreak.